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CRL-2095 CAL 27 人舌鱗癌細胞

簡要描述：CRL-2095 CAL 27 人舌鱗癌細胞，ATCC 細胞|細胞系|細胞株|細胞,細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件。

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CRL-2095 CAL 27 人舌鱗癌細胞

ATCC® Number:CRL-2095™Price:
Designations:CAL 27

Depositors:C Cardona

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens （human）

Morphology:epithelial


Source:Organ: tongue
Disease: squamous cell carcinoma

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Isolation:Isolation date: 1982

Tumorigenic:Yes

DNA Profile （STR）:

Amelogenin: X

CSF1PO: 10,12

D13S317: 10,11

D16S539: 11,12

D5S818: 11,12

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 14,17

CRL-2095 CAL 27 人舌鱗癌細胞



Cytogenetic Analysis:aneuploid; modal number = 43

Age:56 years

Gender:male

Ethnicity:Caucasian

Comments:

CAL 27 cells were resistant to treatment with VDS （vindesine sulfate）, CDP （cis-platinum） or ACTD （actinomycin D）.

A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C

Subculturin


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature

Doubling Time:35 hrs

Related Products:Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002

recommended serum:ATCC 30-2020



References:

23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269























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