日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>CRL-1444A7r5 大鼠胸大動脈平滑肌細(xì)胞

A7r5 大鼠胸大動脈平滑肌細(xì)胞

簡要描述:CRL-1444 A7r5 大鼠胸大動脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-1444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2491

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1444 A7r5 大鼠胸大動脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件

CRL-1444 A7r5 大鼠胸大動脈平滑肌細(xì)胞的詳細(xì)介紹


ATCC® Number:CRL-1444™  Price:$275.00
Designations:A7r5

Depositors:W Carlisle

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:fibroblast

Source:Organ: aorta, thoracic
Strain: DB1X
Tissue: smooth muscle


Cellular Products:myokinase; creatine phosphokinase (muscle isoenzyme); myosin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1444

Applications:transfection host (Roche FuGENE® Transfection Reagents

technology from amaxa)



Age:embryo

Comments:The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase.

Muscle type CPK is synthesized after cell division has ceased.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301

CRL-1444




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产成人精品一区二三区 | 久久中文字幕无码专区| 国产午夜精品一区二区三区软件| 欧美午夜一区二区福利视频| 亚色av| 国产suv精品一区二区6| 另类图区| 玩弄人妻少妇500系列视频| 西欧free性满足hd| 欧洲多毛裸体XXXXX| 少妇熟女久久综合网色欲| 欧美JIZZHD精品欧美| 亲近乱子伦免费视频| 无码av不卡一区二区三区| 久久99精品久久久久久不卡| 欧美老熟妇牲交| 欧美国产成人精品一区二区三区| 久久久久亚洲波多野结衣| 9l国产精品久久久久麻豆| 久久夜色精品亚洲AV三区| 亚洲色偷偷色噜噜狠狠99| 无码人妻精品一区二区三区66| 亚洲 欧美 国产 日韩 精品| 成人国产一区二区三区精品不卡| 日韩AV无码国产精品| 久久久久久久精品免费看| 久久亚洲精品11p| 亚洲国产精品国语在线| 亚洲av成人一区二区三区观看| 国产成人综合精品无码| 色舞月亚洲综合一区二区| 国产AV午夜精品一区二区入口| 欧美特黄特色三级视频在线观看| 四虎国产精品免费永久在线| 夫妻免费无码V看片| 国产精品一区二区无线| 精品国产乱码久久久久久浪潮小说| 亚洲AV成人一区二区三区AV| 精品国产丝袜自在线拍国语| 99久RE热视频这里只有精品6| 久久99精品久久久久久婷婷2021 |