日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2549Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2549
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1412

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2549™    Price:$417.00
Designations:Panc 03.27Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras + (wild-type)Antigen Expression:MHC class I +; MHC class II - [50655] Blood type A; Rh+DNA Profile (STR):Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 8,13 THO1: 6 TPOX: 8,9 vWA: 16,17Age:65 yearsGender:femaleEthnicity:WhiteComments:Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                               Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseDoubling Time:37.5 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-20200.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101Cell culture tested DMSO:ATCC 4-XReferences:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
毛片aaaa| 我和岳乱妇三级3| 女同一区| 手机在线看永久AV片免费| 久久综合给合久久97色| 久久久久久久久毛片精品| 兴化市| 激情内射亚洲一区二区三区爱妻 | 亚洲乱色| 最近免费中文字幕大全免费版视频| 亚洲日韩乱码中文无码蜜桃| 国内精品视频在线观看九九| 色噜噜一区二区三区| 午夜色播| 狠狠操综合| 黄色视频在线免费观看| 久久亚洲AV成人无码软件| 粉嫩虎白女流水白浆在线播放| 久久精品国产精品亚洲精品| 狼人在线二线三线区别大吗 | 狠狠夜夜| www.午夜视频| 白浆网站| 熟女一区二区| 黑人 巨大 迪克 娇小 亚洲女| 涩涩漫画在线观看| 高潮在线| 亚洲va欧美va人人爽成人影院| 成人午夜网| 亚洲乱亚洲乱妇50p| 亚洲av片不卡无码久久| 无码人妻精一区二区三区| 亚洲色欲久久久久综合网| 亚洲AV日韩精品久久久久久 | 东平县| 亚洲综合小说另类图片动图| 爱爱网址| 国产精品久久人妻互换| 成人免费视频网址| 天堂中文在线资| 亚洲色图10p|