日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11005FL62891細(xì)胞, 人肝細(xì)胞

FL62891細(xì)胞, 人肝細(xì)胞

簡要描述:FL62891細(xì)胞, 人肝細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-11005
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-03-26
  • 訪  問  量:1892

產(chǎn)品分類

Product Category

詳細(xì)介紹

FL62891細(xì)胞, 人肝細(xì)胞

ATCC® Number:CRL-11005™    Price:$353.00
Designations:FL 62891

Depositors:ImClone Systems Inc.

Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast

Source:Organ: liver
Cell Type: immortalized with SV40 large T antigen


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
FL62891細(xì)胞, 人肝細(xì)胞

Age:fetus

Comments:The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 32.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 32°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:48 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020



References:70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993
70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994
70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996
70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997
70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998
70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
郎溪县| 大帝av在线一区二区三区| 主人起床尿进奴喉咙里h| 无码人妻久久久一区二区三区免费| 性无码专区一色吊丝中文字幕| 久久18禁高潮出水呻吟娇喘| 性色生活毛片免费旡码| 黄页在线观看| 97久久碰国产精品2023| 亚洲欧美小说| 国产乱理伦片a级在线观看| 欧美一区在线视频| 亚洲av无码国产综合专区| 亚洲AV中文乱码一区二| 亚洲一本一道一区二区三区| 色婷婷综合久久久久中文| 亚洲国产精品久久精品成人网站| 人妻无码一区二区三区免费| 贵德县| 亚洲夜射射夜| 99热精品国产| 成人综合av| 综合网久久| 日韩人妻在线| 精品久久久久久精品| 久久久久久毛片| 在线视频h| 邻居少妇张开腿让我爽视频 | 亚洲精品成人片在线播放| 亚洲av综合久久九九| 末成年女AV片一区二区丫| 欧美最猛黑人XXXX黑人猛交98 | 香蕉精品亚洲二区在线观看| 国产精品内射久久久久欢欢| 大伊香蕉在线面费观看| 精品人伦一区二区三区潘金莲| 91精品国产高清自在线看入口 | 日日噜噜夜夜狠狠视频免费 | 亚洲国产一区二区a毛片| 黄色有几种黄色的颜色| 国产精品乱子伦xxxx裸|