日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動(dòng)物巨噬細(xì)胞>CRL-28433D4/21 豬肺泡巨噬細(xì)胞

3D4/21 豬肺泡巨噬細(xì)胞

簡(jiǎn)要描述:CRL-2843 3D4/21 豬肺泡巨噬細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2843
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:4207

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

ATCC 細(xì)胞|細(xì)胞株|細(xì)胞系|腫瘤細(xì)胞|巨噬細(xì)胞

3D4/21 (ATCC® CRL-2843™)

Organism Sus scrofa, pig

Tissue  lung

Cell Type  macrophage macrophage (alveolar); immortalized with SV40 large T antigen transformed with pSV3-neo

Product Format  frozen

Morphology  macrophage

Culture Properties  adherent

Biosafety Level  2  [Cells contain SV40 viral DNA sequences]

Age  27 days

Gender  unknown

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Strain  Landrace

Applications

These porcine myelomonocytic cell lines may have a wide variety of applications in porcine virology and immunology Ref.



Storage Conditions  liquid nitrogen vapor phase

Derivation  The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.


Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Virus Susceptibility  Bovine adenovirus 3

Classical swine fever virus , Classical swine fever virus

Human parainfluenza virus 3

Swinepox virus

Vesicular stomatitis New Jersey virus

Porcine adenovirus

Herpes simplex virus 1

African swine fever virus

Pseudorabies virus

Vaccinia virus

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Swine vesicular disease virus

Comments

The plasmid carries the genes for neomycin resistance and SV40 large T antigen.


A subpopulation of each cell line (3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844)) was positive, to varying degrees depending on the media formulation, for nonspecific esterase activity and phagocytosis.


Clone 3D4/21 can produce Bovine adenovirus type 3 (BAV-3) to markedly higher titers than clones 3D4/2 and 3D4/31.


Addition of DMSO improved the capability of clone 3D4/21 to replicate the field isolate of African swine fever virus (ASFV/Lillie) compared to the other clones.

Complete Growth Medium  RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Subculturing

Volumes used in this protocol are for 75cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 x 103 to 7 x 103 viable cells/cm2 is recommended.

Incubate cultures at 37°C. Subculture when cell concentration reaches between 3 x 105 and 4 x 105 cells/cm2.

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended

Medium Renewal: Two to three times weekly

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.


CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Cryopreservation

Complete growth medium supplemented with 5% (v

) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: 5% CO2 in air recommended























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
av男人天堂网| 精品国产粉嫩内射白浆内射双马尾| 91l九色丨老熟女专场| 第九色区av天堂| 国产美女自卫慰视频福利| 欧美寡妇xxxx黑人猛交 | 最近免费韩国日本HD中文字幕| 久久亚洲精品国产精品| 无码人妻一区二区无费| 蜜臀久久久精品人妻久久| 99国产揄拍国产精品| 顶级欧美熟妇高潮XXXXX| 免费裸体无遮挡黄网站免费看| 好男人好资源影视在线| 欧美性猛交xxxx乱大交| 绯色蜜臀av性久久久久蜜臀aⅴ| 亚洲色婷婷六月亚洲婷婷6月 | 人妻少妇精品视中文字幕国语| 亚洲精品天天影视综合网| 小欧视频| www.狠狠操| 色呦哟| 欧美牲交videossexeso欧美| 天天做天天爱天天综合网2021| 亚洲日韩中文字幕天堂不卡| av在线亚洲男人的天堂| 吕梁市| 天天色| 日韩精品福利视频| 亚洲在线一区| 99电影| 狠狠色噜噜狠狠狠狠97首创麻豆| 国产成人精品高清在线观看99| 欧美亚洲国产成人一区二区三区| 被闺蜜的男人CAO翻了求饶| 久久综合九色综合欧美| 男女肉粗暴进来120秒动态图| 综合 欧美 亚洲日本| 丰满熟妇乱子伦| 亚洲欧美另类久久久精品 | 日韩av综合|